Required CSV format before you start
Prepare your RNA-Seq count matrix correctly
To use this software, you need to have the RNA-Seq count data after alignment ready in a CSV file where the rows are genes and the columns are samples.
Column structure
The first column should be titled Gene Symbol and should contain gene names. The next columns should represent samples from different groups.
Sample naming rule
The software recognizes samples within a group by the unique name before the parentheses. The number inside parentheses indicates the sample number.
Example with three groups
- Group A has 10 samples.
- Group B has 15 samples.
- Group C has 10 samples.
The sample columns should be named as: A (1), A (2), ..., A (10), B (1), ..., B (15), C (1), ..., C (10).
| Gene Symbol | A (1) | A (2) | B (1) | C (1) |
|---|---|---|---|---|
| GeneA | 120 | 134 | 280 | 95 |
| GeneB | 90 | 88 | 30 | 115 |
Please make sure your CSV file is formatted this way before uploading it. If sample names do not follow the group-name-plus-parentheses pattern, group detection may be incorrect.
